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Image Search Results
Journal: Neural Plasticity
Article Title: Seizure-Induced Regulations of Amyloid- β , STEP 61 , and STEP 61 Substrates Involved in Hippocampal Synaptic Plasticity
doi: 10.1155/2016/2123748
Figure Lengend Snippet: A single ECS but not chronic ECS increases the level of Tyr 876 -phosphorylated GluA2 in the hippocampus. Immunoblot analysis for phosphorylation of GluA2 at Tyr 876 (Y 876 ) or 3Tyr (3Y: Tyr 869 , Tyr 873 , and Tyr 876 ) and total GluA2 expression in the hippocampal crude membrane (P2) fraction following a single ECS ( n = 5 rats per time point) (a–c) and chronic ECS ( n = 5 rats for 72 h time point and n = 6 rats per all other time points) (d–f). The ratio of the phosphorylated GluA2 band intensity over the β -actin band intensity (a-b, d-e) and the ratio of total GluA2 band intensity over the β -actin band intensity (c, f) were calculated per each time point and normalized to that of “no seizure” (NS) sham group. Data shown represent the mean band intensity ± SEM. (a–c) A single ECS increases the level of Tyr 876 -phosphorylated GluA2 at 96 h time point ((b) ∗ p < 0.05) but does not alter the level of 3Tyr-phosphorylated GluA2 and total GluA2. (d–f) Chronic ECS does not change Tyr-phosphorylation of GluA2 (d-e) and total GluA2 expression (f).
Article Snippet: Phosphorylation site specific antibodies used include anti-GluN2B-pTyr 1472 which recognizes phosphorylated Tyr-1472 of GluN2B (P1516-1472, PhosphoSolutions), anti-ERK1/2-pThr 202 /Tyr 204 which recognizes phosphorylated Thr 202 /Tyr 204 of ERK1 and Thr 185 /Tyr 187 of ERK2 (#9106, Cell Signaling), anti-GluA2-p3Y which recognizes phosphorylated Tyr 869 , Tyr 873 , and Tyr 876 (3Y) of GluA2 (#3921S, Cell Signaling), and
Techniques: Western Blot, Expressing